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Growth of human osteoblasts SaOS2 on titanium modified with nanotubes.
Krýslová, Markéta ; Filová, Elena (advisor) ; Melkes, Barbora (referee)
This work summarizes information about the interactions between osteoblasts and nanostructured materials, which are of growing importance and are highly promising in regard to their application in medicine and in tissue engineering. The number of people with artificial replacements of tissues, such as bones, joints, teeth, cartilage, and tendons increases every year. Titanium and his alloys are extensively used for artificial tissue replacements. Titanium is favourable for its mechanical properties that allow the implant to remain in the place of implantation more than thirty years. For better osseointegration the surface of titanium can be modified with hydroxyapatite, coating with diamond-like carbon or plasma spray coating. Another option is to prepare a layer of nanotubes, which forms nanoroughness on material surface. The nanoroughness in turn improves physical and chemical properties of the material surface. Nanostructured materials mimic the natural bone tissue, support adsorption of specific proteins, improve the biocompatibility of the implants and positively influence cell behaviour, e.g. stimulate the synthesis and suitable conformation of specific molecules for cell adhesion and differentiation.
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Biomimetic modifications of titanium in bone tissue engineering.
Krýslová, Markéta ; Filová, Elena (advisor) ; Rampichová, Michala (referee)
When the big joints like a knee or hip joint are damaged, the solution of this problem is an artificial substitute. The replacement of damaged joints with endoprotesis helps to reduce the pain and to move normally. In the design of the implant is necessary to fulfil all requirements on the properties of the material. The surface of implant is important, because it is directly connected to bone tissue. After implantation, the negative effect include infection, inflammation or release of the implant due to limited osseointegration, may appear. The osseointegration can be improved by modifying the material surface. This thesis is focused on development and evaluation of advanced materials imitating the bone structure, especially nanoroughness and the presence of biomimetic component, such as hydroxyapatite. In this study is evaluated adhesion, proliferation, viability, differentiation, and synthesis of specific proteins of human osteoblasts like Saos-2 on titanium modified with nanotubes and plasma sprayed hydroxyapatite compared with smooth surfaces. Key words: titanium, nanotubes, osteoblasts, hydroxyapatite, nanoroughness
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Biomimetic modifications of titanium in bone tissue engineering.
Krýslová, Markéta ; Filová, Elena (advisor) ; Rampichová, Michala (referee)
When the big joints like a knee or hip joint are damaged, the solution of this problem is an artificial substitute. The replacement of damaged joints with endoprotesis helps to reduce the pain and to move normally. In the design of the implant is necessary to fulfil all requirements on the properties of the material. The surface of implant is important, because it is directly connected to bone tissue. After implantation, the negative effect include infection, inflammation or release of the implant due to limited osseointegration, may appear. The osseointegration can be improved by modifying the material surface. This thesis is focused on development and evaluation of advanced materials imitating the bone structure, especially nanoroughness and the presence of biomimetic component, such as hydroxyapatite. In this study is evaluated adhesion, proliferation, viability, differentiation, and synthesis of specific proteins of human osteoblasts like Saos-2 on titanium modified with nanotubes and plasma sprayed hydroxyapatite compared with smooth surfaces. Key words: titanium, nanotubes, osteoblasts, hydroxyapatite, nanoroughness
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Growth of human osteoblasts SaOS2 on titanium modified with nanotubes.
Krýslová, Markéta ; Filová, Elena (advisor) ; Melkes, Barbora (referee)
This work summarizes information about the interactions between osteoblasts and nanostructured materials, which are of growing importance and are highly promising in regard to their application in medicine and in tissue engineering. The number of people with artificial replacements of tissues, such as bones, joints, teeth, cartilage, and tendons increases every year. Titanium and his alloys are extensively used for artificial tissue replacements. Titanium is favourable for its mechanical properties that allow the implant to remain in the place of implantation more than thirty years. For better osseointegration the surface of titanium can be modified with hydroxyapatite, coating with diamond-like carbon or plasma spray coating. Another option is to prepare a layer of nanotubes, which forms nanoroughness on material surface. The nanoroughness in turn improves physical and chemical properties of the material surface. Nanostructured materials mimic the natural bone tissue, support adsorption of specific proteins, improve the biocompatibility of the implants and positively influence cell behaviour, e.g. stimulate the synthesis and suitable conformation of specific molecules for cell adhesion and differentiation.
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